Abstract:

Eight polymorphic di- and tri-nucleotide microsatellite markers are reported for the deep
 sea invertebrate Munida isos from submarine mountains and continental slopes in south
eastern Australia. 140 putative microsatellite sequences were identified in 292 clones
from partial genomic libraries enriched for CA, GA, AAT and CCT motifs. Dinucleotide
loci were hypervariable with 33-43 alleles per locus and expected heterozygosities of 0.94-
0.96. Trinucleotide loci had 21-40 alleles per locus and expected heterozygosities 0.89-
0.96. As with many marine invertebrates, conformity to Hardy Weinberg proportions was
poor suggesting high incidence of null alleles.

These datasets are stored on the GenBank website http://www.ncbi.nlm.nih.gov/genbank/ and referenced in the Technical paper

Species: Munida isos
Number of primers developed: 8
GenBank accession no: HM194717–HM194724
MER database no: 44514–44521
Authors:  Gunasekera, Rasanthi M.; England, Phillip R.; Miller, Karen J.; Audzijonyte, Asta
 

  1. HM194717 Munida isos clone B237 microsatellite sequence
  2. HM194718 Munida isos clone B242 microsatellite sequence
  3. HM194719 Munida isos clone A205 microsatellite sequence
  4. HM194720 Munida isos clone B9 microsatellite sequence
  5. HM194721 Munida isos clone C161 microsatellite sequence
  6. HM194722 Munida isos clone C191 microsatellite sequence
  7. HM194723 Munida isos clone C114 microsatellite sequence
  8. HM194724 Munida isos clone C189 microsatellite sequence

 

 

Document Type: 
Dataset
Availability: 
Available